Studies in immunochemistry: 7. The isolation from hog gastric mucin of the polysaccharide-amino acid complex possessing blood group A specificity.

نویسندگان

  • W T Morgan
  • H K King
چکیده

1. The kinetics of the reaction between HCHO and I-ascorbic acid or chemically related substances have been studied. 2. Indirect evidence is produced for the possible structure of the HCHO-1-ascorbic acid complex. / 3. Lugg's method for determination of I-ascorbic acid has been examined from the point of view of the above experiments, and necessary precautions are stressed. 4. Suggestions for other methods for the determination of I-ascorbic acid in the presence of reductic acid and reductone are advanced.

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منابع مشابه

A spectrophotometric study of blood group A-specific substance isolated from hog gastric mucosa.

The isolation from hog gastric mucosa of homogeneous preparations of the polysaccharide-amino acid complex possessing the ability to inhibit the isoagglutination of human blood group A cells (1) has been hindered not only by dilliculties of manipulation and the presence in gastric mucosa of substances having properties similar to those of blood group A-specific substance, but also by the fact t...

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IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS LXtI Fractionation of Hog and Human A, H, and AH Blood Group Active Substance on Insoluble Immunoadsorbents of Dolichos

The fucose-binding lectins from Lotus tetragonolobus have been purified (1-3), characterized, and shown to be specific for oligosaccharides containing fucosyl residues on C-2 of DGalfll--~4DGlcNAc I (type 2 chains) but not for DGalfll-->3DGlcNAc (type 1 chains) similarly substituted (3, 4). In addition, the Lo~us lectin precipitated with H, A2 and Le a, but failed to interact with A1 or B blood...

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An Examination of the Blood Group a Specific Substance from Hog Gastric Mucin and the Specific Polysaccharide of Bacterium Dysenteriae

a qualitative method for the analysis of the hydrolysis products of complex polysaccharides. Interest especially centres in the general class of mucopolysaccharides-polysaccharides which contain hexosamines and which frequently show a special capacity for forming combinations with proteinsthe reactions of which may be of considerable importance in the storage behaviour of foodstuffs. The specif...

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The estimation of mucin in gastric juice.

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Degradation of blood group antigens in human colon ecosystems. I. In vitro production of ABH blood group-degrading enzymes by enteric bacteria.

Human feces contain enzymes produced by enteric bacteria that degrade the A, B, and H blood group antigens of gut mucin glycoproteins. We have studied their production in fecal cultures to determine if such cultures can be a source for enzyme purification and to explore how blood group antigen-degrading enzymes are adapted in individual human colon ecosystems. They were present in fecal culture...

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عنوان ژورنال:
  • The Biochemical journal

دوره 37 5  شماره 

صفحات  -

تاریخ انتشار 1943